Evaluation of TGF-β1, CCL5/RANTES and sFas/Apo-1 urine concentration in children with ureteropelvic junction obstruction
نویسندگان
چکیده
INTRODUCTION The aim of this study was to evaluate changes in expression of soluble biomarkers tumor factor growth-β1 (TGF-β1), CCL5/RANTES, and sFas/Apo-1 in the urine of patients undergoing ureteropyeloplasty for ureteropelvic junction (UPJ) obstruction. These factors are connected with different processes ongoing in the obstructive uropathy. If their urine concentrations correlate with AP diameter of the renal pelvis and differential function of the affected kidney, they can be helpful in making a decision on corrective surgery. MATERIAL AND METHODS Creatinine, TGF-β1, CCL5/RANTES, and sFas/Apo-1 levels were measured in the urine from the bladder and renal pelvis of 45 patients undergoing ureteropyeloplasty and from bladders of 25 patients undergoing inguinal herniorrhaphy. RESULTS LEVELS OF EXAMINED BIOMARKERS WERE HIGHER IN THE RENAL PELVIS AND BLADDER OF CHILDREN WITH UPJ OBSTRUCTION AS COMPARED TO CONTROLS: TGF-β1 in older children and adolescents (p < 0.05), CCL5/RANTES in the youngest and older children (p < 0.05), and sFas/Apo-1 in all patients (p < 0.05). Twelve months after surgery their levels in the bladder decreased: TGF-β1 in younger and older children (p < 0.05), CCL5/RANTES in the youngest patients and adolescents (p < 0.05), and sFas/Apo-1 in the youngest and older children (p < 0.05). A significant decrease in the AP diameter of the renal pelvis post-operatively (32.09 mm vs. 18.72 mm) (p < 0.01) and significant improvement in renal function (36.94% vs. 42.76%) (p < 0.05) were observed in the examined group. CONCLUSIONS Mean TGF-β1, CCL5/RANTES, and sFas/Apo-1 urine levels are significantly increased in patients with UPJ and decreased 1 year after ureteropyeloplasty. Bladder concentrations of examined factors may be clinically useful markers of obstruction.
منابع مشابه
Results of Pyeloplasty in patients with Ureteropelvic Junction Obstruction in Pediatric Surgery Center in Bandar Abbas
Introduction: Ureteropelvic junction obstruction (UPJO) is associated with inadequate urine drainage from renal pelvis into ureter. UPJO results from incomplete recanalization of proximal ureter during fetal period which finally leads to hydrostatic distention of renal pelvis and calyces. Diagnosis is based on clinical symptoms, ultrasound, and diuretic isotopic renogram. Various surgical metho...
متن کاملEvaluation of Caspase 3 Enzyme and TNF-alpha as Biomarkers in Ureteropelvic Junction Obstruction in Children- a preliminary report
OBJECTIVE To determine the applicability of urinary caspase 3 enzyme and TNF-α as biomarkers in children with ureteropelvic junction obstruction (UPJO). METHODS In this study, 31 unilateral UPJO patients and 33 age- and sex-matched healthy childrens were enrolled. The patients with UPJO consisted of 11 female and 20 male children between the ages of 2 to 62 months old. All participants were e...
متن کاملComparison Between Intravenous Urography and Computed Tomography Urography in Diagnosing Ureteropelvic Junction Obstruction
We read with interest the article by Xie et al. (1) in a previous issue of Nephro-Urology Monthly (October 2011), regarding the comparison between intravenous urography and computed tomography urography in diagnosing ureteropelvic junction obstruction. As previously described, pathological lesions at the ureteropelvic junction are classified into intrinsic and extrinsic abnormalities. In the ca...
متن کاملThe Survey of Fetal Genitourinary Abnormalities
ABSTRACT Congenital genitourinary tract abnormalities occur in 0.1% to 1% of all neonates and are a major cause of perinatal mortality and morbidity. Nowadays, prenatal ultrasonography can detect most malformations and anomalies. In this study the genitourinary tract abnormalities of the Fetuses of the pregnant women by prenatal ultrasonography and causes of these abnormalities were evaluated...
متن کاملOne-Year Follow-Up of Radiologic and Clinical Findings in Children with Prenatal and Neonatal Hydronephrosis
Background & Aims: Hydronephrosis is a common problem in prenatal and newborn infants diagnosed by ultrasonography. Bladder to ureter reflux, the upper or lower urinary tract obstruction, and neurogenic bladder are the most common causes of hydronephrosis in newborns and infants. Methods: In this study, 100 neonates and infants with hydronephrosis were observed clinically and laboratorically fo...
متن کامل